Reference Documentation

Complete reference documentation for OpenHCS configuration formats, syntax specifications, and technical details.

Experimental Configuration Syntax Reference

Configuration References

Experimental Configuration Syntax Reference - Complete syntax reference for Excel-based experimental design configuration

File Format Specifications

Experimental Configuration: - Excel-based configuration format for complex experimental designs - Support for biological and technical replicates - Multi-plate experiment configuration - Control well definition and normalization

Supported Microscope Formats: - ThermoFisher CX5 format (EDDU_CX5) - MetaXpress format (EDDU_metaxpress)

Example Files

Download complete example configuration files:

experimental_config_example.xlsx - Working experimental configuration example

Quick Reference

Essential Configuration Elements:

N                    3                    # Number of biological replicates
Scope               EDDU_metaxpress       # Microscope format

Condition           Drug_Treatment
Dose                0    10   50   100
Wells1              A01  A02  A03  A04    # Biological replicate 1
Plate Group         1    1    1    1

Common Patterns: - Technical replicates: Multiple Wells1 rows for same biological replicate - Multi-plate: Different Plate Group numbers for wells - Controls: Separate Controls block with Group N assignments

See the complete syntax reference for detailed specifications and advanced features.